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Compound feed additive with enriched culture system of Clostridium butyricum, specially developed for laying hens. Probiotics colonize in the gut and continuously produce butyric acid, repairing intestinal mucosa, balancing gut microflora and inhibiting pathogenic bacteria. It improves watery droppings and feed passage, keeps litter firm and dry, reduces dirty eggs and defective eggs. It optimizes nutrient absorption, improves feed-to-egg ratio, stabilizes laying peak and cuts ammonia emission. Green antibiotic-replacement gut health solution for large-scale antibiotic-free layer farming.