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For dairy cattle, the negative energy balance during the perinatal period could cause the accumulation of fat and then comes together with the fatty liver. Liver is the most important metabolic organ for dairy cattle, the deterioration of the liver will cause the metabolic disorders, weakened immunity, and secondary diseases such as ketosis, abomasum displacement, and mastitis. The rumen protected choline chloride that added in the TMR could get into the liver after they were absorbed in the intestines, helping transfer the extra fat then effectively relieve the fatty liver.